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HL-60 tissue was in fact incubated that have MMRI-dos0-PE or isotype control-PE (10?g/ml) with the frost having 20 min Antibody coated cells were then incubated at 37°C/5% CO2 for the indicated times to allow internalisation. After incubation, a secondary GAM IgG-AF488 antibody was applied to the samples for 20 min on ice to detect remaining […]
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